DYKDDDDK Peptide：A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site

產品概述

Synonyms ：DYKDDDDK tag;Flag-tag

同義詞 ：DYKDDDDK 標簽；Flag-tag

Description：

A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide.

描述：

 一種融合標簽，稱為DYKDDDK，由八個氨基酸（Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys）組成，包括一個腸激酶切割位點，專為免疫親和色譜設計。它允許在非變性條件下洗脫。針對該肽的幾個抗體已被開發(fā)出來。一種稱為M1的抗體在雙價金屬陽離子存在下結合該肽，優(yōu)選Ca2+。通過螯合劑實現洗脫。另一種策略是使用過量的游離DYKDDDK肽進行競爭性洗脫。

Sequence：  

Sequence： Shortening DYKDDDDK

Molecular： Formula C₄₁H₆₀N₁₀O₂₀ 

Molecular Weight ：1012.97

產品屬性

Purity ：> 95% 

Solubility ：Soluble in Ultrapure water under 1mg/ml 

Form： Lyophilized 

Storage ：Store at -20°C. 

純度： > 95% 

溶解性 ：可溶于超純水，濃度小于1毫克/毫升

形態(tài) ：凍干粉

儲存 ：溫度保持在-20°C。